The shady areas and rough borders provide habitat for a range of wildflowers and invertebrates. Greater Burnet Saxifrage grows near the motorway bridge as does a patch of Reeds. Common Valerian grows along the track where it borders the grazing fields. The brook adds further interest and supports a number of specialist water plants such as Water Speedwell, Water Crowfoot and Water Starwort. Banded Agrions flit along the brook in the summer months.
This stretch of disused railway line runs between Ratby and Glenfield. Though the original line is broken by an industrial estate a connecting path joins up the two parts. For most of its length it runs parallel with the Rothley Brook and the section that flows under the motorway is included in this site. Much of it is shaded by overhanging trees but in places there is a grassy flora and the bordering field margins add further interest.
| Common Name | Latin Name | Date Recorded | Recorded By |
|---|---|---|---|
| Anomalous Bristle-moss | Orthotrichum anomalum | 14/01/2012 | David Nicholls |
| Physcia aipolia | 14/01/2012 | David Nicholls | |
| Common Flower Bug | Anthocoris nemorum | 09/07/2011 | David Nicholls |
| Ringlet | Aphantopus hyperantus | 09/07/2011 | David Nicholls |
| Honey Bee | Apis mellifera | 09/07/2011 | David Nicholls |
| Fool's Water-cress | Apium nodiflorum | 09/07/2011 | David Nicholls |
| Mugwort | Artemisia vulgaris | 09/07/2011 | David Nicholls |
| Common Water-starwort | Callitriche stagnalis | 09/07/2011 | David Nicholls |
| Common Knapweed | Centaurea nigra | 09/07/2011 | David Nicholls |
| Meadowsweet | Filipendula ulmaria | 09/07/2011 | David Nicholls |
| Liocoris tripustulatus | 09/07/2011 | David Nicholls | |
| Common Bird'sfoot-trefoil | Lotus corniculatus | 09/07/2011 | David Nicholls |
| Ribbed Melilot | Melilotus officinalis | 09/07/2011 | David Nicholls |
| Water Forget-me-not | Myosotis scorpioides | 09/07/2011 | David Nicholls |
| Green-veined White | Pieris napi | 09/07/2011 | David Nicholls |
In Ratby, the start is next to the Railway Pub, though the first stretch of line is then brokem by a road that cuts across.